Fig. 2

Craniofacial Development-Related Genes and miRNAs in Zebrafish Larvae Exposed to 2.5 % CSE. (A) Overlap between differentially expressed genes (DEGs) induced by 2.5 % CSE and genes related to craniofacial development. (B) The volcano plot of DEGs in 2.5 % CSE-exposure and control groups; highlighted genes represent the top 10 intersected craniofacial developmental DEGs with significant expression changes. (C) Protein–Protein Interaction (PPI) network constructed from intersected developmental DEGs using the edge-percolated component (EPC) method; the width and color of each edge (ranging from blue to purple) correspond to the combined score, while the size and color of each node (ranging from yellow to red) relate to the EPC score. (D) Overlap between differentially expressed microRNAs (DEMis) induced by 2.5 % CSE and target miRNAs of intersected developmental DEGs. (E) The volcano plot of DEMis in 2.5 % CSE-exposure and control groups, with highlighted intersected target DEMis. (F) Craniofacial developmental network diagram for miRNA–mRNA interactions. Purple ellipses, upregulated hub craniofacial developmental-related DEGs; red triangles, upregulated target DEMis; and blue 'V' pattern, downregulated target DEMis. (G) Expression patterns of genes and miRNAs as determined by quantitative real-time PCR assay and Illumina sequencing in the craniofacial developmental network.