FIGURE 5

CUT&Tag analysis of histone modifications between stage I oocytes with or without granulosa cells. (A) Heatmap showing the H3K4me3, H3K4me1, H3K27ac, and H3K27me3 enrichment around peak center (peak center ±2 kb) in pure oocytes and oocytes with GCs samples. Peaks were classified into three clusters based on their dynamics between samples: common (present in both samples), pure oocyte-specific (only present in pure oocytes sample), and oocyte with GCs-specific (only present in the mixed sample). The numbers of peaks in each group were labeled. (B) Barplots showing the top 10 GO terms enriched for genes whose TSS were located within ±2 kb of H3K4me3 (upper) or H3K27ac (lower) peaks specific in pure oocytes (left, blue) or mixed samples (right, orange). Oogeneisis and germ cell related terms were highlighted in light blue. (C) H3K4me3, H3K4me1, H3K27ac, and H3K27me3 modifications near differentially GC expressed genes (mafba and rpz5) and oocyte expressed maternal genes (hwa and pou5fa) in stage I oocytes with or without granulosa cells. Promoter of each gene was labeled in light blue. GCs, granulosa cells.