FIGURE

Fig. 2

ID

ZDB-FIG-240404-14

Publication

Lee et al., 2024 - Guarea microcarpa C. DC. extract inhibits NLRP3 inflammasome by suppressing its ATPase activity

Other Figures

All Figure Page

Back to All Figure Page

Fig. 2

GM does not affect NF-κB signaling. (A) LPS-primed J774A.1 cells were treated with GM for 2 h. NF-κB (p65) translocation was confirmed via immunoblotting. (B) pGL4.32 luciferase reporter vector-transfected 293T cells were stimulated with TNF-α (20 ng/mL) and simultaneously treated with GM for 5 h. The luciferase activity of NF-κB was measured using a Promega Bright-Glo luciferase assay system. (C) LPS-primed J774A.1 cells and (D) LPS-primed THP-1 cells were treated with GM for 2 h and activated with nigericin (10 μM) for 30 min or 1 h, respectively. Supernatants and lysates were assessed via immunoblotting. Data are displayed as the means ± SEM. One-way ANOVA and Bonferroni post-hoc tests were used for statistical analyses; *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, n.s. not significant.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Ethnopharmacol.