Fig. 3

The quality of IR² images is robust over large developmental intervals.

a, Representative GFP and IR images of a single z plane in a full 3D stack of Tg(kdrl:GFP) zebrafish larvae at 2, 3 and 4 d after fertilization. Scale bar, 100 µm. dpf, days post fertilization. b, Image reconstructions obtained from IR² models trained with images from 2, 3 and 4 dpf zebrafish larvae. c, Quantification of image quality as measured with the NRMSE and SSIM from the GFP image as well as the images obtained after IR² reconstruction. Values were averaged over all patches extracted from four different samples per age group. Non-transparent violin plots represent the values from the GFP images and the images reconstructed from the IR² network matching the zebrafish age. Transparent violin plots represent reconstruction performed with networks from other developmental stages. Horizontal solid and dashed lines represent the mean and quartile values, respectively.