|
β-catenin stimulates Wnt5b-mediated Tlr4/NFκB activation. a, b β-catenin activates NFκB via Tlr4. a Embryos were injected with control (mKO2) or constitutively active β-catenin mutant (β-cat CA) mRNA, with or without Tlr4 DN mRNA. b Embryos were injected with control or ctnnb2 (zebrafish β-catenin) MO. WISH for dGFP in NFκB-tkP:dGFP-transgenic; frzb in WT embryos at the dome stage, animal view. Scale bar = 200 μm. c NFκB is activated following Wnt/β-catenin activation in the developing dorsal organizer. WISH for dGFP in OTM:d2EGFP and NFκB-tkP:dGFP-transgenic embryos and frzb in WT embryos at the indicated stage. The dGFP-expressing and frzb-expressing dorsal regions are indicated by red arrowheads. Scale bar = 200 μm. d β-catenin activates wnt5b expression in early embryos. Embryos were injected with control (mKO2) or constitutively active β-catenin mutant (β-catCA) mRNA. WISH for wnt5a and wnt5b at the sphere stage, animal view. Scale bar = 200 μm. e, f Wnt/β-catenin signaling activates NFκB via Wnt5b. e Embryos were injected with ctrl or wnt5b MO. f Embryos were injected with control (mKO2) or constitutively active β-catenin mutant (β-cat CA) mRNA, with or without wnt5b MO. WISH for dGFP in NFκB-tkP:dGFP-transgenic embryos at the dome stage, animal view. Scale bar = 200 μm. g, h Wnt5b activates frzb expression through Tlr4/Rel. g Embryos were injected with control (mKO2) or wnt5b mRNA, and co-injected with Tlr4 DN mRNA or rel MO. WISH for dGFP in NFκB-tkP:dGFP-transgenic, frzb in WT embryos at the dome stage, animal view. Scale bar = 200 μm. h WISH for dharma in WT embryos injected with control (mKO2) or wnt5b mRNA at 30% epiboly stage. Scale bar = 200 μm. Box plots of the angle of marker gene show first and third quartile, median is represented by a line, whiskers indicate the minimum and maximum. Each dot represents one embryo. P-values for unpaired two-tailed t-tests are indicated. Source data are provided as a Source Data file.
|
