Figure 3.

Intestinal enterocytes and secretory cells exhibit cell-type-specific transcriptional responses to the microbiota

(A) Cluster 11 is composed of enterocytes from the proximal intestine marked by high expression of rbp2a and chia.2. GO analysis plot represents gene function categories enriched in CVZ versus GF cells in cluster 11 and the dot plot shows genes significantly enriched within CVZ versus GF cells of cluster 11. Genes included in the dot plot designated with an asterisk correspond to genes included in GO categories.

(B) Mucin-secreting cells are localized to cluster 48 indicated by expression of agr2 and muc5.3. GO analysis plot is based on genes significantly enriched within CVZ versus GF cells of cluster 48.

(C) uMAP plots display integration of all agr2+ cells from whole larvae with agr2+ cells from dissected guts. By uMAP and feature plots, subcluster1 displays the most overlap of cells from each experimental group and the highest expression of agr2.

(D) Subcluster 1 demonstrates consistent pairing of cells from each experimental group from the integration.

(E) Dot plot illustrates enriched expression of several genes within CVZ cells of subcluster 1. GO plots in this figure and all following figures use bars to show the total number of genes included in a GO category. The number in parentheses displayed after a GO category title on the y axis signifies the number of GO terms binned into the category. GO terms are considered significant at p < 0.05 after a false discovery rate (FDR) p-corrected adjustment.