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adgrl3.1−/− zebrafish show increased locomotion after 3 days of habituation. A Significant two-way RM ANOVA effect for habituation (F(1.995, 135.7) = 3.84; p* = 0.024) was observed for distance traveled, with no significant effects for interaction between factors (genotype*habituation; F (2, 136) = 1.66; p = 0.19) nor genotype (F (1, 68) = 1.40; p = 0.24). For immobility, a significant effect of genotype*habituation (F (2, 136) = 3.66; p* = 0.03), habituation (F (1.998, 81.51) = 5.92; p* = 0.013), and genotype (F (1, 68) = 10.23; p** = 0.002) was found. Post-hoc analyses showed that adgrl3.1–/– has increased immobility during the first day of habituation compared to WT (p* = 0.018). adgrl3.1−/− also showed a habituation to the novel environment by showing a decrease in their immobility during the second day (p* = 0.025) and third day (p* = 0.03) compared to the first day of habituation. B Representative tracking of a WT vs. adgrl3.1−/− vs. adgrl3.1−/− + ATO animal during the test day. C A significant ANOVA effect was found for distance traveled (F(2,48) = 5.44; p** = 0.007) during the test day. Briefly, distance traveled was increased for adgrl3.1−/− compared to WT animals (p** = 0.005) with no effect for adgrl3.1−/− compared to adgrl3.1−/− + ATO (p = 0.14). No effect for immobility was observed (p = 0.54). The data is represented as mean ± S.E.M.
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