FIGURE 10
- ID
- ZDB-FIG-231002-235
- Publication
- Meier et al., 2023 - Zebrafish as model system for the biological characterization of CK1 inhibitors
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IC50-determination of G2-2 and G2-3 with GST-humCK1δTV1, His-DrCK1δA and His-DrCK1δB. The IWP-derived inhibitors G2-2 and G2-3 were further assessed and IC50-values were determined. The transfer of radioactively labelled γ-phosphate to the substrate α-casein was measured by Cherenkov counting. The inhibitors were applied in different concentrations ranging from 13 to 26667 nM. The standardized kinase- and ATP-concentration as well as reaction time can be found in |