Figure 7.

MR modulates the cortisol-induced reduction in macrophage migration toward a wound site. (A) Number of mpeg+ macrophages that have migrated toward the wound site (200 µm from amputation site), at 4 hours after amputation, in larvae exposed to different levels of cortisol ranging from doses that reflect physiological stress levels (100 ng/mL) to a pharmacological dose (10 μg/mL) (n = 11-30). (B) The number of mpeg+ macrophages that have migrated toward the wound site in the presence of cortisol (10 µg/mL), in combination with either eplerenone (1.25 µM) or mifepristone (1.25 µM) (n = 10-28). (C and D) Transcript abundance of genes encoding the macrophage-specific chemoattractants ccl2 (C) or cxcl11aa (D), determined by qPCR analysis of RNA isolated from zebrafish larvae exposed to cortisol (10 µg/mL) in combination with either eplerenone (1.25 µM) or mifepristone (1.25 µM) (n = 5-6). Bars show mean ± SEM of data pooled from three different experiments (A, B; each data point representing a single larva) or mean ± SEM of 3 individual experiments (C, D). Data were analyzed using a 1-way ANOVA. Statistical significance is indicated by *P ≤ .05, **P ≤ .01, ***P ≤ .001.