Fig. 9

The effects of PTZ-kindling in GPR39 KO or WT (C57BL/6/Tar × CBA/Tar) mice and chronic treatment with TC-G 1008 (10 mg/kg) on the expression of proteins in the GPR39 signaling pathway in the hippocampus. a The PTZ kindling in GPR39 KO or WT (C57BL/6/Tar × CBA/Tar) mice consisted of 14 injections of PTZ (25 mg/kg). The biochemical analyses were performed 24 h after the last PTZ injection. b–d The expression of p-CREB, CREB, BDNF, p-TrkB, and TrkB was normalized to β-actin. The results (means ± SEM) are shown as the p-CREB/CREB, BDNF/β-actin, or p-TrkB/TrkB. b n = 7 in each group. c–d n = 6 in each group. e Representative blots of p-CREB, CREB (~ 46 kDa), BDNF (~ 14 kDa), p-TrkB, TrkB (~ 140 kDa), and β-actin (~ 42 kDa). ^#p-CREB, BDNF, and p-TrkB come from the same blot; ^$CREB and TrkB come from the same blot, thus sharing the corresponding β-actin band. f, h The expression p-ERK1/2, ERK1/2, and SIRT1 was normalized to the total protein concentration. f The results (means ± SEM) are shown as p-ERK1/2/ERK1/2. n = 6 WT VEH, n = 6 WT TC-G 1008, n = 7 KO VEH, n = 7 KO TC-G 1008. h n = 7 WT VEH, n = 6 WT TC-G 1008, n = 5 KO VEH, n = 6 KO TC-G 100. g Representative blots of p-ERK1/2, ERK1/2 (~ 44, 42 kDa) and SIRT1 (the observed band ~ 130 kDa). p values were determined by two-way ANOVA and Bonferroni’s multiple comparison test. *p < 0.05, **p < 0.01