Fig. 3

A Schematic representation of the transplantation procedure. B Survival plots of the irradiated recipients without transplantation (n = 6) or transplanted with KM cells from double transgenic mutant zebrafish (n = 32 for FLT3^ITDIDH2^R140Q, n = 25 for FLT3^ITDIDH2^R172K) or WT(n = 21) 40 days post-primary transplantation. C The percentage of the blast cell in the PB and the representative Wright’s staining of the PB (D) of the primary recipients (n = 6 for all groups) 30 days post-transplantation. E The percentage of the blast cell and the total cell number (F) in the KM and the representative Wright’s staining of the KM (G) of the primary recipients (n = 6 for all groups) 30 days post-transplantation. H Relative mRNA expression of FLT3 and IDH2 in the KM of the primary recipients (n = 3 for all groups). I The size and the representative Wright’s staining of the spleen (J), and the percentage of the blast cell and macrophages in the spleens (K) of the primary recipients (n = 5 for all groups) 30 days post-transplantation. Blast cells and macrophages are indicated by red and black arrows respectively. L Survival plot of the irradiated secondary recipients (n = 16 for FLT3^ITDIDH2^R140Q, n = 15 for others) 40 days post-transplantation. Data are mean ± s.e.m. Student’s t test was performed for C, E, F, H, I, and K (each mutant group vs. WT), **P < 0.01, ***P < 0.001, ****P < 0.0001. Log-Rank test was performed for B and L, *P < 0.05, **P < 0.01, ****P < 0.0001.