Fig. 6

Fig. 6. Synthetic SPM protectin D1 accelerates the macrophage behavioural switch at the wound. (A) Schedule of the experiment. Protectin D1 (PD1) or same volume of ethanol were injected in 3 dpf Tg(mfap4:mCherry-F) larvae, 1 h before the fin fold amputation, and imaged from 5 to 13 hpA. (B) Representative frames of time-lapse imaging of Tg(mfap4:mCherry-F) larvae injected with ethanol as a control (up) or with PD1 (down). White lines indicate wound margin. Scale bars: 100 μm. (C) Quantification of macrophage distance from the wound margin in ethanol - (CTRL) and PD1-injected larvae at 6, 9, and 13 hpA. Number of macrophages is indicated in parenthesis. Mean±sem, from two independent experiments, two-tailed Mann Whitney test, **p < 0.01, ns – not significant. (D) Quantification of macrophage perimeter and circularity in ethanol - (CTRL) and PD1-injected larvae at 6, 9, 10, 11, 12 and 13 hpA. Number of analysed macrophages is indicated in parenthesis; two independent experiments, Mann-Whitney two-tailed test, *p < 0.05, **p < 0.01 and ***p < 0.01.