Fig. 5

Fig. 5. Lipoxygenase 15 activity is required for macrophage behavioural switch (A) Schedule of the experiment. The fin folds of Tg(mfap4:mcherry-F) larvae were amputated at 3 dpf and immediately treated with DMSO or PD146176. Larvae were imaged in live at 6 and 13 hpA. (B) Representative images of the fin folds from amputated Tg(mfap4:mcherry-F) larvae at 13 hpA after DMSO or PD146176 treatment; Maximum projections from confocal microscopy showing mCherry-F macrophages in the fin folds. White lines outline the fin fold and the notochord. Scale bar: 100 μm. (C) Quantification of macrophage wound distance in DMSO- and PD146176-treated larvae at 6 and 13 hpA. Mean±sem, two independent experiments, the number of analysed macrophages from 4 larvae is indicated in parenthesis, two-tailed Mann-Whitney test, *p < 0.05, ns – not significant (D) Quantification of macrophage perimeter and circularity in DMSO- and PD146176-treated larvae at 6 and 13 hpA. Mean±sem, two independent experiments, number of analysed macrophages from 4 larvae is indicated in parenthesis, two-tailed Mann-Whitney test, *p < 0.05, ***p < 0.001. (E) Quantification of the number of recruited macrophages at 6 and 13 hpA, in DMSO- and PD146176-treated larvae. Mean±sem, two independent experiments, number of larvae is indicated in parenthesis. Two-tailed Mann-Whitney test, ns – not significant.