FIGURE 2

dscaml1 deficiency alters the development of CRH^NPO neurons. (A) Illustration of the larval zebrafish brain (lateral view) with rostral to the left. The NPO is highlighted in blue. Imaging planes used for CRH^NPO neuron analysis are shown (light blue planes) (B-D') Developmental trajectory of CRH^NPO neurons, labeled by crhb FISH (green) and co-stained with anti-ERK1/2 (magenta). At each developmental stage, a representative confocal sub-stack containing the NPO is shown, with the NPO (boxed region) enlarged and shown in the right panel without the ERK1/2 co-stain. Wild-type (WT) animals are shown in panels B, C, and (D) dscaml1−/− animals are shown in panels B', C', and D' (E–F) Quantification of the signal intensity per cell (E) and cell number (F). Multiple-comparison corrected p values are as shown. WT: n = 9 (2 dpf), 13 (3 dpf), 15 (5 dpf). dscaml1−/−: n = 9 (2 dpf), 13 (3 dpf), 18 (5 dpf). Scale bars are 20 μm. Mean, standard error, and corrected p values are shown.