FIGURE

FIGURE 2

ID

ZDB-FIG-221109-50

Publication

Xing et al., 2022 - Regulatory factor identification for nodal genes in zebrafish by causal inference

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FIGURE 2

Identification of binding regions in ndr1 and ndr2 loci for Eomesa, β-catenin, and Smad2. Uninjected WT embryos or those injected with 50 pg Myc-eomesa mRNA were harvested at 4.3–5 hpf for chromatin immunoprecipitation using anti-Myc (A and B), anti-β-catenin (C and D), anti-Smad2 (E and F), or IgG antibody (Ab). The immunoprecipitated chromatin was used for quantitative PCR analysis using specific primers targeting different regions, as illustrated. The PCR results were normalized with the input genomic DNA with dilution factor (e.g., 1:10). The x-axis showed the genomic organization of ndr1 or ndr2 with amplified regions numbered. The translation start site was designated as position +1. The y-axis indicated the average percentage (±SEM) of amplified product relative to input DNA (% input) based on two independent experiments. Statistical significance levels: *, p < 0.05; **, p < 0.01; ***, p < 0.001; ns, nonsignificant.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Cell Dev Biol