FIGURE

Fig. 3

ID

ZDB-FIG-221028-18

Publication

Cacialli et al., 2022 - Synergistic prostaglandin E synthesis by myeloid and endothelial cells promotes fetal hematopoietic stem cell expansion in vertebrates

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Fig. 3

The deficiency of slco2b1 induces a decrease of HSPC in the CHT

A
WISH for cmyb expression at 60 hpf in wild‐type and slco2b1 ^−/− embryos.
B
Quantification of cmyb‐expressing cells. Each n represents the number of cmyb‐expressing cells for each embryo (biological replicates). Each experiment has been repeated three independent times.
C
Schematic indicating the imaging area in the tail at 60 hpf, as indicated by the black box; fluorescence imaging in the CHT of kdrl:mCherry;cmyb:GFP embryos injected with control‐ and slco2b1‐Mos.
D
Quantification of HSPCs associated with ECs. Each n represents the number of yellow spots for each embryo (biological replicates). Each experiment has been repeated three independent times.
E
Experimental outline and quantification of time‐lapse live imaging in controls and slco2b1‐morphants cmyb:GFP ⁺ cells, using Imaris software.

Data information: Center values denote the mean, and error values denote s.e.m. The statistical analysis was completed using an unpaired two‐tailed t‐test. ***P < 0.001. Scale bar is 500 μm (A); 200 μm (C).

Source data are available online for this figure.

Expression Data

Genes:	GFP myb
Fish:	slco2b1^{sa37367/sa37367} s896Tg/s896Tg; zf169Tg/zf169Tg s896Tg/s896Tg; zf169Tg/zf169Tg + MO2-slco2b1
Knockdown Reagent:	MO2-slco2b1
Anatomical Term:	hematopoietic multipotent progenitor cell
Stage:	Pec-fin

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	slco2b1^{sa37367/sa37367} s896Tg/s896Tg; zf169Tg/zf169Tg s896Tg/s896Tg; zf169Tg/zf169Tg + MO2-slco2b1
Knockdown Reagent:	MO2-slco2b1
Observed In:	blood vessel endothelium blood vessel endothelium hematopoietic multipotent progenitor cell hematopoietic multipotent progenitor cell
Stage:	Pec-fin

Phenotype Detail

Acknowledgments

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