FIGURE

Fig. 4

ID

ZDB-FIG-221028-19

Publication

Cacialli et al., 2022 - Synergistic prostaglandin E synthesis by myeloid and endothelial cells promotes fetal hematopoietic stem cell expansion in vertebrates

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Fig. 4

The defect in HSPCs proliferation in slco2b1‐deficient embryos can be rescued by PGE2 treatment

A
Anti‐GFP and pH3 immunostainings of either controls (Ctrl‐Mo) or slco2b1‐morphants (slco2b1‐MO) cmyb:GFP embryos.
B
Quantification of the number of the pH3+ HSCs in controls or slco2b1‐morphants. Center values denote the mean, and error values denote s.e.m, statistical analysis was completed using an unpaired two‐tailed t‐test. ***P < 0.001.
C
Experimental outline to measure PGE2 level by ELISA kit in control and slco2b1‐MO at 60 hpf.
D
Quantification of PGE2 concentration in control‐ and slco2b1‐morphants. The statistical analysis was completed using an unpaired two‐tailed t‐test *P < 0.01. Center values denote the mean, and error bars denote s.e.m.
E
Fluorescence imaging in the CHT of cmyb:GFP embryos injected with control‐ and slco2b1‐MOs and treated with PGE₂.
F
Quantification of GFP‐positive cells. Statistical analysis: one‐way ANOVA, multiple comparison, *P < 0.01; ****P < 0.0001. Center values denote the mean, and error bars denote s.e.m. Data information: Scale bar is 50 μm (A); 200 μm (E).

Source data are available online for this figure.

Expression Data

Gene:	GFP
Fish:	zf169Tg/zf169Tg zf169Tg/zf169Tg + MO2-slco2b1
Condition:	chemical treatment by environment: prostaglandin E2
Knockdown Reagent:	MO2-slco2b1
Anatomical Term:	caudal hematopoietic tissue hematopoietic multipotent progenitor cell
Stage:	Pec-fin

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	zf169Tg/zf169Tg + MO2-slco2b1
Condition:	chemical treatment by environment: prostaglandin E2
Knockdown Reagent:	MO2-slco2b1
Observed In:	caudal hematopoietic tissue hematopoietic multipotent progenitor cell caudal hematopoietic tissue hematopoietic stem cell proliferation prostaglandin-E synthase activity
Stage:	Pec-fin

Phenotype Detail

Acknowledgments

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