FIGURE

Fig. 5

ID

ZDB-FIG-220905-44

Publication

Iyer et al., 2022 - A lysosomal regulatory circuit essential for the development and function of microglia

Other Figures

All Figure Page

Back to All Figure Page

Fig. 5

Fig. 5. Simultaneous mutation in tfeb, tfe3a, or tfe3b rescues flcn and rraga mutant phenotypes.

(A) NR assay and quantification of microglia in larvae obtained from flcn^+/−; tfeb^+/−; tfe3b^+/− intercross. (B and C) LysoTracker Red assay and quantification of the area of LysoTracker Red punctae in microglia of larvae from (B) rraga^+/−; tfe3a^+/− intercross and (C) flcn^+/−; tfeb^+/−; tfe3b^+/− intercross. Images show LysoTracker Red signal in dorsal view of the midbrain. Scale bars, 50 μm. Graphs show mean + SD; significance was determined using parametric unpaired t test. (D) apoe mRNA expression at 4 dpf and rescue of microglia in the progeny of rraga^+/−; tfe3a^+/− intercross and flcn^+/−; tfeb^+/−; tfe3b^+/− intercross. (E) mpeg:GFP expression to visualize microglia and macrophages in flcn; tfeb; tfe3b triple mutants. Arrows denote microglia and macrophages in which ramified morphology has been restored, asterisks denote cells with amoeboid morphology, and insets show magnified views of cell morphology. Scale bars, 50 μm. (F) Quantification of amoeboid morphology and rescue. Graph shows mean + SD; significance was determined using nonparametric Mann-Whitney U test. The number of animals analyzed for each experiment is listed in the tables below each graph; all the panels are representative of at least two independent experiments.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Sci Adv