FIGURE

Fig. 6

ID

ZDB-FIG-220516-37

Publication

Sojan et al., 2022 - Probiotics Enhance Bone Growth and Rescue BMP Inhibition: New Transgenic Zebrafish Lines to Study Bone Health

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Fig. 6

Figure 6. BMP inhibitor exposure followed by two probiotics treatments in Tg(col10a1a:col10a1a-GFP) larvae. (a) Integrated pixel intensity values for various areas measured in 10 dpf zebrafish Tg(col10a1a:col10a1a-GFP) larvae divided into various groups—control, DMSO, LDN, LDN+BS and LDN+LL—and stained with AR. DMSO was used as additional control since it was employed as the solvent for LDN. One-way ANOVA and Tukey’s multiple comparison tests were used, and statistical significance was set at p < 0.05. Different letters denote statistically significant differences among the experimental groups. (b) GFP, AR staining and merged images of the head area (lateral and ventral views) of Tg(col10a1a:col10a1a-GFP) larvae of the different treated groups. White arrows denote GFP in various bony structures and the white arrowhead indicates the presence of a signal in additional structures (that was absent in other groups) in BS-treated fish after LDN exposure (LDN + BS).

Expression Data

Gene:	GFP
Fish:	col10a1a^ulg072Tg
Conditions:	chemical treatment by environment: 5-(6-(4-(Piperazin-1-yl)phenyl)pyrazolo[1,5-a]pyrimidin-3-yl)quinoline bacterial treatment by exposure to environment: Bacillus subtilis, chemical treatment by environment: 5-(6-(4-(Piperazin-1-yl)phenyl)pyrazolo[1,5-a]pyrimidin-3-yl)quinoline bacterial treatment by exposure to environment: Lactococcus lactis, chemical treatment by environment: 5-(6-(4-(Piperazin-1-yl)phenyl)pyrazolo[1,5-a]pyrimidin-3-yl)quinoline
Anatomical Term:	whole organism
Stage:	Days 7-13

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	col10a1a^ulg072Tg
Conditions:	chemical treatment by environment: 5-(6-(4-(Piperazin-1-yl)phenyl)pyrazolo[1,5-a]pyrimidin-3-yl)quinoline bacterial treatment by exposure to environment: Bacillus subtilis, chemical treatment by environment: 5-(6-(4-(Piperazin-1-yl)phenyl)pyrazolo[1,5-a]pyrimidin-3-yl)quinoline bacterial treatment by exposure to environment: Lactococcus lactis, chemical treatment by environment: 5-(6-(4-(Piperazin-1-yl)phenyl)pyrazolo[1,5-a]pyrimidin-3-yl)quinoline
Observed In:	head bone mineralization whole organism
Stage:	Days 7-13

Phenotype Detail

Acknowledgments

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