ZFIN ID: ZDB-FIG-220509-6
Blackwell et al., 2022 - Hnrnpul1 controls transcription, splicing, and modulates skeletal and limb development in vivo. G3 (Bethesda)   12(5) Full text @ G3 (Bethesda)
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Term:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Observed In:
Stage: Protruding-mouth

Fig. 2.

Loss of hnrnpul1l and hnrnpul1 leads to differential splicing. a) Schematic showing the processing of hnrnpul1l to form standard transcript and AS of exon 12 of the hnrnpul1l gene as a result of CRISPR-Cas9 targeted mutagenesis. b) PSI for exon 12 of hnrnpul1l in wild type and hnrnpul1/1l mutant embryos at 3 dpf. c) Change in PSI of all exon skipping events in hnrnpul1/1l mutant embryos compared to wild types. d) Detailed view of PSI of genes associated with phenotypes, points represent each biological replicate. e) Change in percent intron retention (PIR) of all intron retention events in hnrnpul1/1l mutant embryos compared to wild types. f) Detailed view of PIR of genes associated with phenotypes, points represent each biological replicate. Details of affected exon/intron are given in Supplementary Table 1. g) Volcano plot showing all differentially expressed genes. Gray points = 1>Log2 FC>-1 or P > 0.05, orange points = 1<Log2 FC<-1 and P ≤ 0.05, green points = 1<Log2 FC<-1 and P ≤ 0.01. h) qPCR validation hnrnpul1 and hnrnpul1l expression in hnrnpul1/1l mutants at 3 dpf compared to wild types. **P ≤ 0.01, ****P ≤ 0.0001, determined by Student’s t-test.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
hnrnpul1 WT standard conditions Protruding-mouth whole organism RTPCR
hnrnpul1ca54/ca54; hnrnpul1lca52/ca52 standard conditions Protruding-mouth whole organism RTPCR
hnrnpul1l WT standard conditions Protruding-mouth whole organism RTPCR
hnrnpul1ca54/ca54; hnrnpul1lca52/ca52 standard conditions Protruding-mouth whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
hnrnpul1ca54/ca54; hnrnpul1lca52/ca52 standard conditions Protruding-mouth whole organism hnrnpul1 expression decreased amount, abnormal
Protruding-mouth whole organism hnrnpul1l expression decreased amount, abnormal
Protruding-mouth whole organism alternative mRNA splicing, via spliceosome increased process quality, abnormal
Acknowledgments:
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