Figure 5

Figure 5. Injection of recombinant Pgk1-Flag into the brain could partially rescue the impairment of swimming performance of MPTP-induced zebrafish larvae. Six groups of zebrafish larvae at 6 dpf, as indicated, were categorized: (1) DsRed (2dpf): embryos at 2 dpf were injected with DsRed protein without MPTP treatment and served as a negative control; (2) MPTP + DsRed (2dpf): embryos at 2 dpf were injected with DsRed protein and treated with MPTP as a mock control; (3–5) Experimental groups: (3) MPTP + Pgk1 (2 dpf): embryos at 2 dpf were injected with Pgk1 and treated with MPTP from 2 to 6 dpf; (4) MPTP + Pgk1 (4 dpf): embryos at 4 dpf were injected with Pgk1 and treated with MPTP from 2 to 6 dpf; and (5) MPTP + Pgk1 (2 + 4 dpf): embryos at 2 and 4 dpf were individually injected with Pgk1 and treated with MPTP from 2 to 6 dpf; and (6) MPTP + Pgk1-T378P (2 + 4 dpf): embryos at 2 and 4 dpf were individually injected with mutated Pgk1-T378P and treated with MPTP from 2 to 6 dpf. The swimming route of each zebrafish larva at 6 dpf was tracked using ImageJ software, and its swimming distance was calculated in cm. (A) The three times head touch-evoked swimming routes of a single larva in six groups of zebrafish larvae were recorded. (B) Statistical analysis of the total swimming distance (in cm) per larva in each group. The total distance of a single larva was the sum of swimming distance instigated by touches to the head and averaged from three trials. The total distance of each experiment was the average total distance obtained from 20 to 35 larvae, while that of each group was averaged from three independent experiments. Student’s t-test was used to determine significant differences between each group (***, p < 0.001).