Figure 2

Figure 2. Distribution of the injected exogenous DsRed protein in brain chamber. We injected exogenous red fluorescence protein (DsRed) into the brain chamber of a zebrafish embryo at (A,B) 2-, (C–F) 4-dpf, and (G–J) a 6-month-old adult zebrafish. (A) The glass needle represents the injection site of zebrafish embryo’s head at 2 dpf. (C) Schematic diagram depicts the direct injection of DsRed into the brain chamber of zebrafish embryo at 4 dpf. The glass needle marked in red represents the injection site. (D) Dorsal view of 4-dpf embryo injected with DsRed under bright-field microscopy; (E) Lateral view of 4-dpf embryo injected DsRed under fluorescence microscopy; (F) Dorsal view of 4-dpf embryo injected DsRed under fluorescence microscopy. (G) Schematic diagram depicts a small incision that was generated above the optic tectum of adult zebrafish by a 30-gauge syringe. (H) Dorsal view of adult zebrafish injected DsRed by glass needle under bright-field microscopy; (I) Schematic diagram depicts the distribution of injected DsRed in the brain through the cerebroventricular fluid. (J) Dorsal view of adult zebrafish injected DsRed after 30 min under fluorescence microscopy. ob: olfactory bulb, tel: telencephalon, ot: optic tectum, c: cerebellum. Scale bar: (A–F) 25 µm; (G–J) 500 µm.