Fig. 4.

Mobility patterns of YFP-H-Ras and its mutants, YFP-H-Ras^V12 and YFP-H-Ras^N17, in epidermal cells of the zebrafish embryos. (A) Fraction size of the fast-diffusing population (α), plotted against the time lag. (B) Mean squared displacements plotted against the time lag for the fast-diffusing fraction (r²₁). (C) Mean squared displacements plotted against the time lag for the slow-diffusing fraction (r²₂). Results of the fits are summarized in Table 1. To establish the values of dynamic parameters, three different embryos per each H-Ras genetic mutant (YFP-H-Ras, YFP-H-Ras^V12 and YFP-H-Ras^N17) were imaged on each of the three different experimental days. Each data point is presented in the form of a mean±s.e.m., and the 95% c.i. of the mathematical fit is shown. Shapiro–Wilk statistical test was performed to check for normality of the data set. Statistical analysis was performed, using a one-way ANOVA [P(α)>0.05 and P(r²₁,r²₂)<0.05 at a t_lag of 25 ms] with a Tukey's range post-hoc test (for details of the post-hoc test results, see Table 2). (D-F) Caterpillar plots presenting the effect range of a different experimental day, different embryos within an experimental day, and different areas within an embryo to an overall variability in the fast-diffusing fraction size (D), mean squared displacement of the fast-diffusing fraction (E) and mean squared displacement of the slow-diffusing fraction (F). Effect ranges represent the relative deviation of group intercepts from the overall mean with 95% c.i. Red lines indicate the overall mean of the data, while black points denote groups that significantly deviate from the overall mean among experimental days, individual embryos and areas within an embryo. The data points are sorted based on their deviation from the total average, beginning with the ones most negatively deviating from the overall mean. The values in D were logit transformed, and the values in E and F were logarithm transformed, to meet the statistical hypotheses of the hierarchical linear model. MSD, mean squared displacement.