FIGURE

Figure 1

ID

ZDB-FIG-220302-78

Publication

Vagionitis et al., 2022 - Clusters of neuronal neurofascin prefigure the position of a subset of nodes of Ranvier along individual central nervous system axons in vivo

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Figure 1

Localization of transgenic node of Ranvier reporters along unmyelinated and myelinated axon stretches

(A) Schematic of node of Ranvier reporter constructs used.

(B) Confocal images of nodal reporters along individually labeled myelinated axons in transgenic animals that have all myelin labeled. Scale bar, 2 μm. (B′) Fluorescence intensity traces of each reporter around the node. Bold lines represent mean, shaded areas SD; n = 3/3 (Nfasca), 4/3 (Cntn1a), and 5/4 (NaV-II-III) animals/technical replicates.

(C) Individually partially myelinated axon at 8 dpf co-expressing cntn1b:Nfasca-EYFP in a full transgenic myelin reporter background. (C′) Magnification of the boxed area in (C). Arrowheads indicate Nfasca-EYFP clusters along unmyelinated axon parts. Arrows point to heminodal Nfasca-EYFP. Scale bars, 10 μm.

(D) Fluorescence intensity quantifications at different regions. Median ± IQR (Kruskal-Wallis test with multiple comparisons); n = 7/7 animals/technical replicates.

See also Figure S1 for supporting information.

Expression Data

Genes:	Cerulean YFP
Fish:	tum101Tg; ue601Tg
Anatomical Terms:	myelin accumulating cell neuron node of Ranvier
Stage:	Days 7-13

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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