Figure 5

Effect of anticancer drugs on the growth of human uveal melanoma 92.1-RFP⁺/luc⁺ xenografts. (A) Effect of paclitaxel, panobinostat, and everolimus treatments on the proliferation of 92.1-RFP⁺/luc⁺ cells in vitro. Viable cells were counted after 48 h of incubation with increasing concentrations of paclitaxel or panobinostat or after 72 h of incubation with everolimus. Data are the mean ± SEM of two independent experiments. (B) After 92.1-RFP⁺/luc⁺ cell grafting into the zebrafish eye, 0.4 pmoles/embryo of paclitaxel, panobinostat, everolimus or the corresponding volume of DMSO were injected in the same eye. Tumor growth was evaluated at t₄ by measuring the cell luminescence signal in the lysates of the whole embryos. Data are the mean ± SEM of two independent experiments. Each dot represents one embryo. * p < 0.05 and *** p < 0.001 vs. DMSO, Student’s t-test. (C) 3D reconstruction of the eye region of 92.1-RFP⁺/luc⁺ xenografts evaluated 4 days post implantation in the absence or in the presence of paclitaxel injection. Scale bar: 50 µm. Asterisk indicates the superficial ocular vasculature; CVP, choroidal vascular plexus.