Figure 6—source data 1.

Zebrafish retinal pigment epithelium (RPE) flattening is associated with an abrupt decrease of cell proliferation.

(A) Confocal images of dorsally viewed Tg(E1-bhlhe40:GFP) embryos exposed to 5-bromo-2′-deoxyuridine (BrdU) at different developmental stages as indicated in the panel and immunostained for BrdU (magenta) and GFP (green). (B) Percentage of RPE proliferating cells (BrdU+/total Hoechst +) in 17–48 hpf Tg(E1-bhlhe40:GFP) embryos. Mean ± SD; n = 5 embryos per stage. Scale bar: 100 µm.

Quantification of the RPE proliferating cells at diffrent developmental stages as reported in <xref rid='fig6' ref-type='fig'>Figure 6B</xref>.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
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