FIGURE 2

Yap inhibition reduces cell proliferation in the retina after photoreceptor-induced light lesion. (A–F) Transverse cryosections of DN-Yap− (A–C) and DN-Yap+ (D–F) retinas immunostained for proliferating cell nuclear antigen (PCNA) (magenta) and Zpr-1 (green) and counterstained with DAPI (gray). White arrowheads and arrows indicate PCNA+ cells in the inner nuclear layer (INL) and outer nuclear layer (ONL), respectively, at 1 dpl (A,D magnified, Ai,Di), 2 dpl (B,E, magnified, Bi,Ei), and 3 dpl (C,F, magnified, Ci,Fi). (G) Quantification of PCNA + cells in DN-Yap− and DN-Yap + larva retinas from 1 to 3 dpl, in the INL and ONL. (H) Quantification of EdU + cells in DN-Yap− and DN-Yap + larva retinas from 1 to 3 dpl, in the INL and ONL. ns, non-significant; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Unpaired t-test with Welch’s correction. Asterisks delimitate the lesioned region. Dashed lines delimitate INL from ONL. White boxes delimitate magnified (Ai–Fi). Scale bars correspond to 50 μm in (A,C), and 20 μm in magnified (Ai,Ci).