FIGURE

Figure 4

ID
ZDB-FIG-210919-26
Publication
Qi et al., 2021 - Zebrafish BID Exerts an Antibacterial Role by Negatively Regulating p53, but in a Caspase-8-Independent Manner
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Figure 4

Apoptotic activity of zebrafish Bid at the early apoptosis stage (A) and the later apoptosis stage (B). EPC cells were transiently transfected with a 2 μg p3xFLAG or Bid-FLAG plasmid. At 24 hours post-transfection, the cells were infected with E. ictaluri with an MOI of 1, 5, and 10 at 28°C. After infection for 1 h, the extracellular bacteria were killed using gentamicin (50 µg/mL). Apoptosis at 6 hpi was detected using an Annexin V-FITC/PI Apoptosis Detection Kit on the CytoFLEX LX flow cytometer. *p< 0.05.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
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