ZFIN Image: Qi et al., 2021, Figure 4

Image

Figure Caption

Figure 4

Apoptotic activity of zebrafish Bid at the early apoptosis stage (A) and the later apoptosis stage (B). EPC cells were transiently transfected with a 2 μg p3xFLAG or Bid-FLAG plasmid. At 24 hours post-transfection, the cells were infected with E. ictaluri with an MOI of 1, 5, and 10 at 28°C. After infection for 1 h, the extracellular bacteria were killed using gentamicin (50 µg/mL). Apoptosis at 6 hpi was detected using an Annexin V-FITC/PI Apoptosis Detection Kit on the CytoFLEX LX flow cytometer. *p< 0.05.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Immunol