Figure 5
Figure 5 Antibacterial activity of zebrafish Bid in vitro (A) and in vivo (B). For in vitro bacterial invasion assays (A), the EPC cells were transiently transfected with 500 ng p3×FLAG or Bid-FLAG plasmid. At 24 h post-transfection, the cells were infected with E. ictaluri at an MOI of 10. At 1.5 h post-infection, the extracellular bacteria were killed using gentamicin (50 µg/ml). At 3 and 6 hpi, the cells were lysed, and the intracellular bacterial colony-forming units (CFU) were calculated. For in vivo bacterial invasion assays (B), the fertilized eggs were microinjected with 200 ng/μl p3×FLAG or Bid-FLAG. At 4 dpf, the larvae were infected with E. ictaluri at an MOI of 10. At 48 hpi, the fish were ground, and the CFU was calculated. *p < 0.05, **p < 0.01. |