PUBLICATION
Zebrafish BID Exerts an Antibacterial Role by Negatively Regulating p53, but in a Caspase-8-Independent Manner
- Authors
- Qi, Z., Yan, D., Cao, L., Xu, Y., Chang, M.
- ID
- ZDB-PUB-210918-24
- Date
- 2021
- Source
- Frontiers in immunology 12: 707426 (Journal)
- Registered Authors
- Cao, Lu, Chang, Mingxian
- Keywords
- BID, Edwardsiella ictaluri, antibacterial activity, apoptosis, p53
- MeSH Terms
-
- Animals
- Apoptosis Regulatory Proteins/metabolism*
- Caspase 8
- Edwardsiella ictaluri
- Enterobacteriaceae Infections/immunology*
- Fish Diseases/immunology*
- Gene Expression Regulation
- Tumor Suppressor Protein p53/metabolism*
- Xenopus Proteins/metabolism*
- Zebrafish/immunology*
- PubMed
- 34531858 Full text @ Front Immunol
Citation
Qi, Z., Yan, D., Cao, L., Xu, Y., Chang, M. (2021) Zebrafish BID Exerts an Antibacterial Role by Negatively Regulating p53, but in a Caspase-8-Independent Manner. Frontiers in immunology. 12:707426.
Abstract
Bid (BH3-interacting domain death agonist), a member of the Bcl-2 family, plays a crucial role in the initiation of apoptosis. Independent of its apoptotic function, Bid is also involved in the regulation of inflammation and innate immunity. However, the role of Bid during bacterial pathogen infection remains unclear. In the present study, Bid of zebrafish (Dario rerio) was cloned and its functions during Edwardsiella ictaluri infection were investigated. Zebrafish Bid enhances the apoptosis rate of Epithelioma papulosum cyprini (EPC) cells following E. ictaluri infection. Importantly, in vitro and in vivo bacterial invasion assays showed that overexpressed Bid could significantly inhibit the invasion and proliferation of E. ictaluri. Real-time qPCR analysis revealed that p53 gene expression was downregulated in embryos microinjected with Bid-FLAG. Further, in vitro and in vivo bacterial invasion assays showed that overexpressed p53 increased the invasion and proliferation of E. ictaluri. Moreover, the invasion and proliferation of E. ictaluri were inhibited when co-overexpressing Bid and p53 in vivo and in vitro. Further, the numbers of E. ictaluri in larvae treated with Z-IETD-FMK (caspase-8 inhibitor) were higher than those of larvae without Z-IETD-FMK treatment, while the number of E. ictaluri in larvae microinjected with bid-Flag decreased significantly, even if the larvae were treated in advance with Z-IETD-FMK. Collectively, our study demonstrated a novel antibacterial activity of fish Bid, providing evidence for understanding the function of apoptosis associated gene in pathogen infection.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping