FIGURE 2

HRAS^G12V expressing PNCs show increased proliferation and altered morphology from 24 hpi. (A) Representative confocal image showing EdU labeling (Magenta) in skin cells expressing eGFPCAAX (Green) at 24 hpi. (B) Representative confocal image showing EdU labeling (Magenta) in skin cells expressing eGFPHRAS^G12V(Green) at 24 hpi. Yellow arrows indicate EdU^+ve eGFP^+ve cells, scale bar = 20 μm. (C) Graph showing increased proliferation in PNCs compared to eGFPCAAX expressing healthy skin cells 12 hpi (p = 0.057, n = 10); 24 hpi (p = 3.602e-011, n = 10), 36 hpi (p = 1,021e-07, n = 10), unpaired t-test with Holm-Sidak method for correction of multiple comparisons. (D) Assessing cell morphology shows that from 24 hpi, a significant proportion of PNCs have lost the normal polygonal shape and become elongated or developed long filopodia (one-way ANOVA with Tukey’s multiple comparisons test, ****p < 0.0001).