Figure 6—figure supplement 2.

Treatment of medaka-derived organoids with the GSK3β inhibitor CHIR-99021 promote RPE differentiation.

(a) Schematic representation of CHIR-99021 treatment. Day 1 aggregates were washed and incubated with Matrigel for 6 hr. Organoids were washed and incubated in media containing 5 μM CHIR-99021 or DMSO. (b) DMSO- and CHIR-99021-treated day 2 organoids and medaka embryonic eye (42 hr post-fertilization [hpf]) showing expression of retina- and RPE-specific transcription factors Rx2 and Otx2, respectively. (c) DMSO- (n = 6/6) and CHIR-99021-treated (n = 13/13) day 4 organoids, including section through CHIR-99021-treated organoid stained with anti-Otx2 antibody. Dashed lines indicate retinal or RPE domains. re, retina; le, lens; RPE, retinal pigmented epithelium. Scale bars: 100 μm, 50 μm in enlargements in b and in fish embryonic retina in b.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
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