PUBLICATION
Fish primary embryonic pluripotent cells assemble into retinal tissue mirroring in vivo early eye development
- Authors
- Zilova, L., Weinhardt, V., Tavhelidse, T., Schlagheck, C., Thumberger, T., Wittbrodt, J.
- ID
- ZDB-PUB-210714-17
- Date
- 2021
- Source
- eLIFE 10: (Journal)
- Registered Authors
- Wittbrodt, Jochen
- Keywords
- developmental biology, in vitro organogenesis, medaka, organoids, regenerative medicine, retina, stem cells, zebrafish
- MeSH Terms
-
- Animals
- Cell Differentiation
- Embryonic Stem Cells/cytology*
- Embryonic Stem Cells/metabolism
- Humans
- Morphogenesis
- Organogenesis*
- Organoids/cytology*
- Organoids/metabolism
- Oryzias
- Pluripotent Stem Cells/physiology
- Retina/cytology*
- Retina/growth & development
- Retina/metabolism
- Zebrafish
- PubMed
- 34252023 Full text @ Elife
Citation
Zilova, L., Weinhardt, V., Tavhelidse, T., Schlagheck, C., Thumberger, T., Wittbrodt, J. (2021) Fish primary embryonic pluripotent cells assemble into retinal tissue mirroring in vivo early eye development. eLIFE. 10:.
Abstract
Organoids derived from pluripotent stem cells promise the solution to current challenges in basic and biomedical research. Mammalian organoids are however limited by long developmental time, variable success, and lack of direct comparison to an in vivo reference. To overcome these limitations and address species-specific cellular organization, we derived organoids from rapidly developing teleosts. We demonstrate how primary embryonic pluripotent cells from medaka and zebrafish efficiently assemble into anterior neural structures, particularly retina. Within 4 days, blastula-stage cell aggregates reproducibly execute key steps of eye development: retinal specification, morphogenesis, and differentiation. The number of aggregated cells and genetic factors crucially impacted upon the concomitant morphological changes that were intriguingly reflecting the in vivo situation. High efficiency and rapid development of fish-derived organoids in combination with advanced genome editing techniques immediately allow addressing aspects of development and disease, and systematic probing of impact of the physical environment on morphogenesis and differentiation.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping