Fig. 4

Effect of phloroglucinol (PG) and dieckol (DK) on the formation of tube-like structures in EA.hy926 cells cultured on Matrigel and treated with a high concentration of glucose. (A) Cells were incubated in a medium containing 1% penicillin/streptomycin for 12 h. Thereafter, the cells were replated on Matrigel-coated plates at a density of 5 × 103 cells/well. After 8 h, images of the cultures were captured using phase-contrast microscopy (4 × magnification). (a) 0 mM glucose + 0 µM PG; (b) 30 mM glucose + 0 µM PG; (c) 30 mM glucose + 0.8 µM PG; (d) 30 mM glucose + 2.4 µM PG; and (e) 30 mM glucose + 8 µM PG; Angiogenic score of the effect of PG on EA.hy926 cells treated with a high concentration of glucose. (B) (a) 0 mM glucose + 0 µM DK; (b) 30 mM glucose + 0 µM DK; (c) 30 mM glucose + 0.1 µM DK; (d) 30 mM glucose + 0.3 µM DK; and (e) 30 mM glucose + 1 µM DK; Angiogenic score of the effect of DK on EA.hy926 cells treated with a high concentration of glucose. The effect of 30 mM of glucose was compared to that of B; blank (0 mM glucose + 0 μM PG, DK), ### p < 0.001. Percentage gap closure was normalized to that of C: control (30 mM glucose + 0 μM PG, DK); ns, not significant, * p < 0.05, *** p < 0.001.