PUBLICATION
Phloroglucinol and dieckol isolated from Ecklonia cava suppress impaired diabetic angiogenesis; A study of in-vitro and in-vivo
- Authors
- Hwang, J., Yang, H.W., Lu, Y.A., Je, J.G., Lee, H.G., Fernando, K.H.N., Jeon, Y.J., Ryu, B.
- ID
- ZDB-PUB-210323-18
- Date
- 2021
- Source
- Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 138: 111431 (Journal)
- Registered Authors
- Keywords
- Angiogenesis, Diabetes, Dieckol, EA.hy926 cells, Phloroglucinol, Zebrafish
- MeSH Terms
-
- Angiogenesis Inhibitors/isolation & purification
- Angiogenesis Inhibitors/pharmacology
- Angiogenesis Inhibitors/therapeutic use*
- Animals
- Animals, Genetically Modified
- Benzofurans/isolation & purification
- Benzofurans/pharmacology
- Benzofurans/therapeutic use*
- Diabetes Mellitus/chemically induced
- Diabetes Mellitus/drug therapy*
- Diabetes Mellitus/metabolism
- Dose-Response Relationship, Drug
- Endothelial Cells/drug effects*
- Endothelial Cells/metabolism
- Glucose/toxicity
- Humans
- Phaeophyceae*
- Phloroglucinol/isolation & purification
- Phloroglucinol/pharmacology
- Phloroglucinol/therapeutic use*
- Protein Structure, Tertiary
- Zebrafish
- PubMed
- 33752058 Full text @ Biomed. Pharmacother.
Citation
Hwang, J., Yang, H.W., Lu, Y.A., Je, J.G., Lee, H.G., Fernando, K.H.N., Jeon, Y.J., Ryu, B. (2021) Phloroglucinol and dieckol isolated from Ecklonia cava suppress impaired diabetic angiogenesis; A study of in-vitro and in-vivo. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie. 138:111431.
Abstract
Abnormalities in angiogenesis that are associated with diabetes may contribute to vascular complications and result in disabilities and death. Furthermore, an imbalance in angiogenesis in different tissues, including the retina and kidney, can play a role in the pathogenesis of diabetic microvascular complications. Phlorotannins, such as phloroglucinol (PG) and dieckol (DK), which are found in Ecklonia cava exhibit antioxidant and anti-inflammatory activities that improve endothelial function in hypertension. However, reports on the effects of these compounds on diabetes-induced angiogenesis in vivo and in vitro are scarce. In this study, we assessed the antiangiogenic effects of PG and DK on endothelial cells treated with a high concentration of glucose to mimic angiogenesis. In addition, we sought to determine the effects of these compounds on cell proliferation, cell migration, and capillary formation. In silico docking of PG and DK into VEGFR-2 revealed their potential as therapeutic agents against angiogenesis. Further, both compounds were identified to inhibit the formation of the retinal vessel in transgenic zebrafish (flk:EGFP) embryos under high glucose conditions. These findings suggested that PG and DK derived from E. cava are potential inhibitors of angiogenesis in diabetic vascular complications and could, therefore, be used to develop angiogenic agents.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping