FIGURE

F

ID

ZDB-FIG-210409-115

Publication

Acin-Perez et al., 2020 - A novel approach to measure mitochondrial respiration in frozen biological samples

Other Figures

All Figure Page

Back to All Figure Page

Lysates from fresh and frozen tissues maintain intact electron transport system

Representative pyruvate + malate seahorse profile using the standard respirometry protocol in mouse liver homogenates obtained from fresh or frozen tissue. Pyruvate + malate using the standard respirometry protocol. Pyruvate + malate + ADP (PMA), oligomycin (oligo), FCCP, and antimycin A + rotenone (AA + ROT)

MRR dependent by pyruvate + malate in fresh and frozen liver homogenates.

Representative succinate + rotenone seahorse profile using the standard respirometry protocol in liver homogenates obtained from fresh or frozen tissue.

MRR dependent by succinate + rotenone in fresh and frozen liver homogenate.

Representative NADH seahorse profile using the standard respirometry protocol in liver homogenates obtained from fresh or frozen tissue.

MRR dependent by NADH in fresh and frozen liver homogenate.

Data information: Panels (A, C, and E) are representative seahorse traces including four technical replicates. Biological replicates: (B), n = 6; (D), n = 8; and (F), n = 4. Every biological replicate represents the average of four technical replicates. Data are the mean ± SEM.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ EMBO J.