FIGURE

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ID

ZDB-FIG-210409-117

Publication

Acin-Perez et al., 2020 - A novel approach to measure mitochondrial respiration in frozen biological samples

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<styled-content toggle='no' style='fixed-case'>RIFS</styled-content> is compatible with a variety of different tissues

A–E

Representative seahorse profiles using RIFS respirometry protocol in frozen homogenates from mouse BAT (A), heart (B), kidney (C), brain (D), and lung (E).

BNGE and Western blot of soleus muscle incubated with trypsin prior to homogenization.

BNGE followed by Western blot of soleus muscle incubated with collagenase type II prior to homogenization.

SDS–PAGE followed by Western blot of soleus muscle incubated with collagenase type II prior to homogenization.

RIFS respirometry quantification in soleus muscle in the indicated conditions.

Complex I‐, II‐, and IV‐dependent respiration in homogenates from frozen tissues from wild‐type mice using RIFS respirometry protocol per milligram of protein.

Data information: Biological replicates: (A–E, I and J), n = 3–6. Every biological replicate represents the average of four technical replicates. Data are the mean ± SEM. Western blots illustrate a representative blot of three independent experiments. Source data are available online for this figure.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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