ZFIN Image: Acin-Perez et al., 2020, F

Image

Figure Caption

F Lysates from fresh and frozen tissues maintain intact electron transport system

A

Representative pyruvate + malate seahorse profile using the standard respirometry protocol in mouse liver homogenates obtained from fresh or frozen tissue. Pyruvate + malate using the standard respirometry protocol. Pyruvate + malate + ADP (PMA), oligomycin (oligo), FCCP, and antimycin A + rotenone (AA + ROT)

B

MRR dependent by pyruvate + malate in fresh and frozen liver homogenates.

C

Representative succinate + rotenone seahorse profile using the standard respirometry protocol in liver homogenates obtained from fresh or frozen tissue.

D

MRR dependent by succinate + rotenone in fresh and frozen liver homogenate.

E

Representative NADH seahorse profile using the standard respirometry protocol in liver homogenates obtained from fresh or frozen tissue.

F

MRR dependent by NADH in fresh and frozen liver homogenate.

Data information: Panels (A, C, and E) are representative seahorse traces including four technical replicates. Biological replicates: (B), n = 6; (D), n = 8; and (F), n = 4. Every biological replicate represents the average of four technical replicates. Data are the mean ± SEM.

Acknowledgments

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