FIGURE

Fig. 1

ID

ZDB-FIG-210322-18

Publication

Leijten et al., 2021 - Thermal Proteome Profiling in Zebrafish Reveals Effects of Napabucasin on Retinoic Acid Metabolism

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Fig. 1

Graphical representation of the workflow for zebrafish thermal proteome profiling. Zebrafish embryo lysates were treated with either DMSO (control) or napabucasin before being subjected to heat treatment. If the drug binds to a protein, the complex can be stabilized leading to an increase in melting point. By quantification of the remaining soluble protein amount, melting curves can be generated. Phenotypic screening of zebrafish embryos using the same drug was done in parallel, which allows for direct correlation of the biological effects to the molecular drug targets.

Expression Data

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Antibody Labeling

Phenotype Data

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Mol. Cell. Proteomics