ZFIN Image: Leijten et al., 2021, Fig. 1

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Figure Caption

Fig. 1

Graphical representation of the workflow for zebrafish thermal proteome profiling. Zebrafish embryo lysates were treated with either DMSO (control) or napabucasin before being subjected to heat treatment. If the drug binds to a protein, the complex can be stabilized leading to an increase in melting point. By quantification of the remaining soluble protein amount, melting curves can be generated. Phenotypic screening of zebrafish embryos using the same drug was done in parallel, which allows for direct correlation of the biological effects to the molecular drug targets.

Acknowledgments

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