Fig. 3

Fig. 3. Migration of GFP⁺ NC cells ventrally in GFP− host chicken after the insertion of the GFP⁺ neural fold. A: A schematic graph illustrating the insertion of GFP⁺ neural fold to GFP− host chicken embryo. B: Photomicrographs of a GFP⁺/GFP⁻ chicken chimera at 1 DPS. Top: fluorescent image to show GFP signals; Bottom: merged fluorescent and bright-field images. C: Single-plane laser scanning confocal images of a section (C₁) from the position indicated by white line in B and higher power images (C₂) from the area indicated by dashed square shown in C^{1. Sections were immunoreacted for NC cell marker HNK1 (magenta). Scale bars: 500 ​μm in B; 80 ​μm in C₁ and 20 ​μm in C₂.}