PUBLICATION

Taste buds are not derived from neural crest in mouse, chicken, and zebrafish

Authors
Yu, W., Wang, Z., Marshall, B., Yoshida, Y., Patel, R., Cui, X., Ball, R., Yin, L., Kawabata, F., Tabata, S., Chen, W., Kelsh, R.N., Lauderdale, J.D., Liu, H.X.
ID
ZDB-PUB-201217-12
Date
2020
Source
Developmental Biology   471: 76-88 (Journal)
Registered Authors
Ball, Rebecca, Chen, Wenbiao, Kelsh, Robert, Lauderdale, James D.
Keywords
Chicken, Mouse, Neural crest, Progenitors, Taste buds, Zebrafish
MeSH Terms
  • Animals
  • Cell Lineage*
  • Chick Embryo
  • Chickens
  • Mice
  • Mice, Transgenic
  • Neural Crest/cytology
  • Neural Crest/embryology*
  • Taste Buds/cytology
  • Taste Buds/embryology*
  • Zebrafish
PubMed
33326797 Full text @ Dev. Biol.
Abstract
Our lineage tracing studies using multiple Cre mouse lines showed a concurrent labeling of abundant taste bud cells and the underlying connective tissue with a neural crest (NC) origin, warranting a further examination on the issue of whether there is an NC derivation of taste bud cells. In this study, we mapped NC cell lineages in three different models, Sox10-iCreERT2/tdT mouse, GFP+ neural fold transplantation to GFP- chickens, and Sox10-Cre/GFP-RFP zebrafish model. We found that in mice, Sox10-iCreERT2 specifically labels NC cell lineages with a single dose of tamoxifen at E7.5 and that the labeled cells were widely distributed in the connective tissue of the tongue. No labeled cells were found in taste buds or the surrounding epithelium in the postnatal mice. In the GFP+/GFP- chicken chimera model, GFP+ cells migrated extensively to the cranial region of chicken embryos ipsilateral to the surgery side but were absent in taste buds in the base of oral cavity and palate. In zebrafish, Sox10-Cre/GFP-RFP faithfully labeled known NC-derived tissues but did not label taste buds in lower jaw or the barbel. Our data, together with previous findings in axolotl, indicate that taste buds are not derived from NC cells in rodents, birds, amphibians or teleost fish.
Genes / Markers
Figures
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Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes