FIGURE

Fig. 4

ID

ZDB-FIG-210126-13

Publication

Fuhrmann et al., 2020 - Genetic developmental timing revealed by inter-species transplantations in fish

Other Figures

All Figure Page

Back to All Figure Page

Fig. 4

Topological organisation of retinal ganglion cells in ectopic retina. (A-A‴) DAPI and immunostaining using an anti-Rx2 antibody on a medaka retina cryosection. Retinal ganglion cells localise to the ganglion cell layer (GCL) and Rx2+ photoreceptors (cyan staining in A′-A‴) are found in the outer nuclear layer (ONL), adjacent to the retinal pigmented epithelium (black region in the transmitted channel, A‴). (B-C″) DAPI staining of whole-mount retinae in zebrakas shows conspicuous layering (arrows in B,B′) and clusters of retinal ganglion cells (arrows in B″,B‴) labelled in green using a Tg(atoh7:EGFP) zebrafish donor. Single plane (C) and stack (C′,C″) showing RGCs and their axons (arrow in C′) in an ectopic zebraka retina (n=6 retinae in six chimeras). Scale bars: 100 µm in A,A′,B-C″; 20 µm in A″,A‴. GCL, ganglion cell layer; INL, inner nuclear cell layer; ONL, outer nuclear cell layer; CMZ, ciliary marginal zone; RPE, retinal pigmented epithelium; D, donor; H, host.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development