ZFIN ID: ZDB-PUB-201011-1
Genetic developmental timing revealed by inter-species transplantations in fish
Fuhrmann, J.F., Buono, L., Adelmann, L., Morales, J.R.M., Centanin, L.
Date: 2020
Source: Development (Cambridge, England)   147(22): (Journal)
Registered Authors: Centanin, Lazaro
Keywords: Developmental timing, Genetic chimera, Inter-species transplantation, Lens induction, Medaka, Organogenesis, Retina, Retina-tectal projection, Zebrafish
Microarrays: GEO:GSE150009
MeSH Terms:
  • Animals
  • Blastula*/embryology
  • Blastula*/transplantation
  • Heterografts
  • Oryzias/embryology*
  • Retina/cytology
  • Retina/embryology*
  • Transplantation Chimera/embryology*
  • Zebrafish/embryology*
PubMed: 33033120 Full text @ Development
The path from a fertilised egg to an embryo involves the coordinated formation of cell types, tissues and organs. Developmental modules (Raff, 1996) comprise discrete units specified by self-sufficient genetic programs that can interact among each other during embryogenesis. Here we took advantage of the different span of embryonic development between two far related teleosts, zebrafish (Danio rerio) and medaka (Oryzias latipes), of 3 and 9 days respectively, to explore modularity principles. We report that inter-species blastula transplantations result in the ectopic formation of a retina formed by donor cells - a module. We show that the developmental time of the retina follows a genetic program: an ectopic zebrafish retina in medaka develops with zebrafish dynamics. Heterologous transplantation results in a temporal decoupling between the donor retina and host organism, illustrated by two paradigms that require retina-host interactions: lens recruitment and retino-tectal projections. Our results uncover a new experimental system to address temporal decoupling along embryonic development, and highlight the presence of largely autonomous but yet interconnected developmental modules orchestrating organogenesis.