FIGURE

Fig. 7

ID

ZDB-FIG-210126-16

Publication

Fuhrmann et al., 2020 - Genetic developmental timing revealed by inter-species transplantations in fish

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Fig. 7

Ectopic zebrafish retina in zebrakas recruits the lens from the medaka host. (A) Transcriptomes of medaka (upper, n=2), zebrafish (middle, n=2) and zebraka (bottom, n=3) plotted along the zebrafish genome. Lens zebrafish genes (crystalin a and crystalin b a2a: cryaa and cryba2a, respectively) are expressed in zebrafish but not in zebrakas. (B-B″) Lateral views of a zebraka where donor Tg(OlBactin2:CAAX-EGFP) cells were transplanted into a Tg(crya:ECFP) host. Merged (B) and single-channel (EGFP in B′, ECFP in B″) images. Cyan expression in the lens of the ectopic retina reveals its host origin (n=3 transplantation events, n=7 chimeras). (C-C″) Ventral views of a medrafish at 5 dpf, where donor Tg(ubiq:H2B-EGFP)(crya:ECFP) cells were transplanted into a non-labelled host. Merged (C) and single-channel (EGFP in C′, ECFP in C″) images. Cyan expression in the lens of the ectopic retina reveals its donor origin (n=10 transplantation events, n=23 chimeras). Scale bars: 100 µm. D, donor; H, host.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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