FIGURE

Fig. 7.

ID

ZDB-FIG-210108-59

Publication

Carroll et al., 2020 - An Irf6-Esrp1/2 regulatory axis controls midface morphogenesis in vertebrates

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Fig. 7.

ANC of esrp1/2 double mutants is populated by undifferentiated cells. Representative z-stacks of RNAscope ISH of coronal sections of esrp1/2 double CRISPR mutants and WT clutch-mate controls at 4 dpf. (A) Sections through ANC anterior to the eyes. col2a1 (red) staining depicts normal morphology of the ANC cartilage elements in WT, while a cleft is apparent in the esrp1/2^−/− zebrafish, with dapi (blue)-stained cells between adjacent trabeculae (arrowheads). These col2a1^– cells do not express epithelial markers krt4 (cyan) or krt5 (magenta), except around the periphery. (B) Sections posterior to those in A show col2a1^– cells continuing inferior to the trabeculae in the esrp1/2 mutant zebrafish, and cells have low expression of irf6 (boxed area). (C) Zoomed image of col2a1^– cells from the boxed area in B, showing irf6 expression (green). Dashed lines outline ANC cartilage elements. Scale bars: 50 μm.

Expression Data

Genes:	col2a1a irf6 krt4 krt5
Fish:	TU esrp1^fb401/fb401; esrp2^fb402/fb402 (TU)
Anatomical Terms:	cranial cartilage anterior region neurocranium cell oral cavity epithelial cell
Stage:	Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	esrp1^fb401/fb401; esrp2^fb402/fb402 (TU)
Observed In:	neurocranium chondrocyte
Stage:	Day 4

Phenotype Detail

Acknowledgments

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