FIGURE

Figure EV2

ID

ZDB-FIG-201107-18

Publication

Costa et al., 2020 - RAB13 mRNA compartmentalisation spatially orients tissue morphogenesis

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Figure EV2

<styled-content toggle='no' style='fixed-case'>CRISPR</styled-content>‐Cas9 editing of the <italic><styled-content toggle='no' style='fixed-case'>RAB</styled-content></italic> 3′<styled-content toggle='no' style='fixed-case'>UTR</styled-content><italic>in vitro and in vivo</italic> does not generate off‐target mutations

Chromatogram confirming the excision of the LE within RAB13 3′UTR in HUVECs.

List of predicted CRISPR‐Cas9 off‐target genes and RNAseq mismatch detection in CRISPR‐Cas9-derived HUVEC clones (n = 1 each genotype).

Chromatogram confirming the CRISPR‐Cas9-mediated excision of 482‐nt within the rab13 3′UTR in zebrafish embryos.

List of predicted CRISPR‐Cas9 off‐target genes and RNAseq mismatch detection in Tg(kdrl:EGFP) rab13^+/+ and rab13^{∆3′UTR/∆3′UTR} embryos (n = 2 each genotype).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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