Figure 3

Kcnj13 is ectopically expressed in somites of the Dhi2059 mutant. Whole-mount in situ hybridizations of zebrafish embryos at the stages from 15S to 48 hpf were performed. WT embryos (A, D–F, and J). kcnj13^Dhi2059/+ fish embryos (B, C, G–I, and K). (A) Kcnj13 is expressed at the pnd in WT embryos. (B) In the kcnj13^Dhi2059/+ mutant, kcnj13 is expressed in the somites at the 15S stage. (C) Dorsal view of the embryo in (B). (D–F) At the 24-hpf stage, kcnj13 is continually expressed in the pronephric ducts of WT embryos. (D) Lateral view. (E) Ventral view of the 24-hpf embryos. The dashed line indicates the approximate position of the transverse section in (F). (G–I) The expression of kcnj13 in the kcnj13^Dhi2059/+ mutant at the 24-hpf stage. The kcnj13 mRNA is also expressed in the somites in addition to pnds. (H) Ventral view of the same embryo in (G). The dashed line indicates the position of the transverse section in (I). (I) Transverse section revealed that kcnj13 is expressed in the dm. (J) At the 48-hpf stage, kcnj13 is continually expressed in pnds in WT fish embryos, and it is also expressed in melanocytes (arrowheads). (K) In the kcnj13^Dhi2059/+ fish embryos, kcnj13 expression is similar to WT embryos at 48 hpf. The somite expression is no longer detectable. Bar, 250 μm (A–E, G, H, J, and K). Bar, 100 μm (F and I). 15S, 15-somite; dm, dermomyotome; hpf, hours postfertilization; mRNA, messenger RNA; n, notochord; nt, neural tube; pnd, pronephric ducts; so, somite, WT, wild-type.