ZFIN ID: ZDB-FIG-200814-1
Tao et al., 2020 - LIN28B regulates transcription and potentiates MYCN-induced neuroblastoma through binding to ZNF143 at target gene promotors. Proceedings of the National Academy of Sciences of the United States of America   117(28):16516-16526 Full text @ Proc. Natl. Acad. Sci. USA
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Term:
Stage: Adult
PHENOTYPE:
Fish:
Observed In:
Stage: Adult

Fig. 1 Overexpression of WT or mutant LIN28B increases the penetrance of MYCN-induced neuroblastoma. (A and B) Schematic of the WT (A, LIN28B_WT) and mutant (B, LIN28B_MU) human LIN28B proteins. CSD; NoLS, nucleolar localization signal; CCHC zinc-finger domain; NLS, nuclear localization signal. Five point mutations (arrowheads) were created in the LIN28B_MU. (C–H) Lack of detectable EGFP expression in the IRG of a 17-wpf EGFP transgenic fish (C) and 39-wpf LIN28B_WT (D) and LIN28B_MU (E) fish; and EGFP expression in the tumors (arrows) arising from the IRG of 17-wpf EGFP;MYCN (F), LIN28B_WT;MYCN (G) and LIN28B_MU;MYCN (H) fish. (Scale bar, 1 mm.) (I) Kaplan–Meier analysis of cumulative frequency of neuroblastoma in transgenic lines. Statistical analysis was performed using the logrank test. (J and K) The relative expression of zebrafish let-7b (J) and let-7f (K) miRNAs in EGFP;MYCN (M), LIN28B_WT;MYCN (WT), and LIN28B_MU;MYCN (MU) tumors by qRT-PCR. Five independent tumors from 6-mo-old fish were analyzed for each group. Each dot represents the expression levels of the indicated gene normalized to U6 snRNA. Horizontal bars indicate means ± SD. Statistical analysis was performed using the two-tailed unpaired t test. ***P < 0.001.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
dre-let-7b zdf16Tg standard conditions Adult interrenal gland neuroblastoma RTPCR
zdf16Tg; zdf39Tg standard conditions Adult interrenal gland neuroblastoma RTPCR
zdf16Tg; zdf40Tg standard conditions Adult interrenal gland neuroblastoma RTPCR
mirlet7f zdf16Tg standard conditions Adult interrenal gland neuroblastoma RTPCR
zdf16Tg; zdf39Tg standard conditions Adult interrenal gland neuroblastoma RTPCR
zdf16Tg; zdf40Tg standard conditions Adult interrenal gland neuroblastoma RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
zdf15Tg standard conditions Adult interrenal gland morphology, normal
zdf16Tg standard conditions Adult interrenal gland neuroblastoma neoplastic, abnormal
zdf16Tg; zdf39Tg standard conditions Adult interrenal gland neuroblastoma dre-let-7b expression decreased amount, abnormal
Adult interrenal gland neuroblastoma mirlet7f expression decreased amount, abnormal
Adult interrenal gland neuroblastoma neoplastic, exacerbated
zdf16Tg; zdf40Tg standard conditions Adult interrenal gland neuroblastoma neoplastic, exacerbated
zdf39Tg standard conditions Adult interrenal gland morphology, normal
zdf40Tg standard conditions Adult interrenal gland morphology, normal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA